Western Blotting Protocols

Methods described at this site:

  • Transfer to PVDF
  • Transfer to nitrocellulose and alkaline phosphatase detection (BioRad)
  • Transfer to nitrocellulose and alkaline phosphatase detection (OWL)
  • Chemiluminescent detection
  • Staining blots to detect proteins
  • Fast Green
  • Coomassie Blue