This site contains several abbreviated protocols (or instructions) have been developed and used in our laboratory and those that were developed during the summer course "Ecophysiology and Molecular Biology of Marine Macrophytes (Hopkins Marine Station, Pacific Grove CA)." I thank all of the students who road tested the protocols. These pages should be used as guidelines and all readers are recommended to review the primary sources or manufacturers' instructions. Please contact me if you would like a current citation for the method (debrobertson@clarku.edu).

Protocols:

• Molecular Biology
  • DNA extraction from marine phytoplankton
  • DNA extraction from marine algae and seagrasses
  • RNA extraction from diatoms using RNeasy kit
  • RNA extraction from diatoms using glass beads and RNeasy Kit
  • RNA extraction from red algae
  • cDNA synthesis using Qiagne's Quantitech RT kit
  • Cloning PCR products

  ---

• Protein Analysis
  • SDS-PAGE
  • Western Blotting
  • Silver Stain
  • BioRad Bradford Assay
  • Purification of His-tagged Proteins

• Physiological/Biochemical Methods
  • Photosynthesis
  • Pigments
  • Cell counting using a hemacytometer
  • Enzyme Assays

  ---

General Information:

• Common Laboratory Solutions
• Chemical concepts