This extraction method is based on previously published protocols (Doyle and Doyle, 1987) and works well for a variety of marine phytoplankton. The method yeilds DNAsuitable for many molecular applications including PCR and Southern blotting. The DNA can be further purified using the methods described by Coyer et al., 1994.
2x CTAB Buffer
- 100 mM Tris pH 8.0
- 1.4 M NaCl
- 20 mM EDTA
- 2% CTAB
- 0.1% PVPP
- Add beta-mercaptoethanol to 0.2% (v/v) daily.
- 10 mM Tris (pH 8.0)
- 0.1 mM EDTA
Coyer, J. A., Robertson, D. L. & Alberte, R. S. (1994). Genetic variablity within a population and between diploid/haploid tissue of Macrocystis pyrifera (Phaeophyceae). Journal of Phycology 30, 545-542.
Doyle, J. J. & Doyle, J. L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytoch. Bull. 19, 11-15.