Materials
Transect
Dive weight
Pencil
Slate
Thermometer
Gorgonian Key
Key to Hard Corals
Methods
Site Selection
Main Study
Wells and North
Site Selection
Three locations were chosen for
intensive study based on different physical properties: John Smith bay,
Whalebone Bay, and Castle Harbor. Two more locations were studied with
less accurate methods; Well’s Bay and North Rock. Well’s bay was
information was collected in too short of a time scale to conduct the full
methods and North Rock was too deep to run a transect.
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Main study - Intensive site
Transect Placement
At each location we arrived on site
and recorded location name, day, time, weather, and tide conditions.
We entered the water carrying a
measuring tape with the end attached to a dive weight, a pencil, a
thermometer, and a slate for recording data.
The
area was surveyed, and a location for a transect was chosen based on
habitat heterogeneity. The goal was to pick an area that was a
representative sample of the whole bay (as we had explored it). Site
placement was only limited by depth, it could not be too deep because the
survey was done by diving and swimming along the transect, which became
too difficult when deeper then about 5 meters (especially with the wave
action at the shore).
The dive weight was dropped at one
end of the chosen transect location and the measuring tape was unraveled
in a straight line. Transect lines were limited in length due to two
factors, the tape had to stay straight and weighted on the floor and the
unweighted end of the transect had to be snuggled under some rock/dead
coral formation at the far end. As happy accident would have it, it has
been shown that regional species richness is accurately calculated at
transects that are as small as 10 meters long, and the minimum transect
length in this study was 8 meters long (Karlson and Cornell 2000). This
was the only transect below 10 meters in length and the average length was
27.4 ± 14.3 meters. Length of the transect could be variable because the
data was standardized as density per meter squared during statistical
analysis.
Collecting Data
Coral species were counted along
the right side of the measuring tape in a meter wide transect. The taxa
included were: Diploria strigosa, D. labyrinthiformis,
Porites astreoides, Montastaea fanki. Gorgonia ventailna,
Pseudopterogorgia sp, Briareum asbestinum,
Plexaurella
sp. Pamphlets from the BBSR was used for taxonomic identification (Bilewich
2007).
Step 6 was repeated for the left
side of the transect (to maximize time and transect numbers), with a
slight offset from the tape so that individuals found directly under the
tape were carefully omitted from this count.
Special Adjustment: Castle Harbor
Causeway
The Castle Harbor
causeway site was too shallow and too sparsely populated to conduct
meaningful studies with the shorter transects. Instead, a swim by method
was conducted, counting individuals in about a meter wide line of vision
for a certain number of kicks. Kicks were timed three separate times
along a transect (3.33 kicks constituted 1 meter).
The
count data was transformed to density measures in an excel spreadsheet.
Data Analysis
Density measures were compared
between locations for each taxon recorded (excluding Pseudopterogorgia
sp, Montastaea fanki, because they were not found in the where
transects were placed in the three intensive study sites) using ANOVA and
subsequent Tukey-Kramer tests. The ANOVA determined if there was
variation in density distribution for the coral taxon between the three
locations and the Tukey-Kramer test determined what locations accounted
for the difference (are all three different, are two the same, which
two?).
For each location a Shannon
evenness index (SHEI) was conducted to determine overall species richness
and evenness among all taxa present. SHEI was conducted using average
percent cover of all transects measured in the location.
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Wells Bay and North Rock
The same species were counted, but
measured in percent cover over the entire area surveyed. The area
surveyed was a subset along an imaginary transect that was about 15 meters
long. The percentage was a rough estimate based on observation.
The percent cover was used in a
SHEI to characterize the sites, but could not be compared empirically to
the density derived data due to lack of accuracy.