Biochemistry and Molecular Biology

Timothy Lyerla

Timothy A. Lyerla

Professor of Biology
Department of Biology
Biochemistry and Molecular Biology Program
Clark University
15 Maywood St.
Worcester, MA 01610-1477

Office: #342; Lab: #353
Phone: (508) 793-7521
Email: tlyerla@clarku.edu


Read Prof. Lyerla's CV
Tim Lyerla's Lab Pages

Current Research and Teaching

Professor Lyerla has retired from teaching full time, but maintains a research laboratory at Clark and teaches occasionally on a course-by-course basis. Research in my laboratory concerns the expression of mutant and variant characters in established and primary cell cultures from vertebrate organisms. The haploid cell line from Rana pipiensembryos, ICR2A, is used for basic studies from an established line, while cultures of lung cell macrophages from mice that serve as animal models for Hermansky-Pudlak (HPS) and Chediak-Higashi Syndromes(CHS) are used for primary cell culture work.

Vertebrate cell culture is an important tool for basic and biomedical studies, and some mastery of the techniques used for the research is needed in order to probe the molecular basis of questions concerning chromosome stability in vitro, the development of drug resistance, mutant effects on secretory pathways leading to serious genetic disorders. My laboratory is concerned with each of these issues, and using cultured cells to explore them in debth with specific goals as to possible outcomes and future research.

Selected Publications

Lyerla, T.A. 2007. Scientific detectives solve ashen mutant problem. Blood 109 (4): 1.

Lyerla, T.A., M. Borchers, G. Jahreis, J. Tan, P. Ohtake, E.K. Novak, R.T. Swank. 2003. Abberant lung structure, composition and function in a murine model of Hermansky-Pudlak syndrome. Am. J. Physiol. Lung Cell Mol. Physiol. 285(3): L643-L653.

Delprato, A., S. Raghavan,T.A. Lyerla. 2000. An established light ear mutant (C57BL/6J-Pdeb (rd1) le) mouse cell line exhibits a block to secretion of lysosomal enzymes. Exp. Cell. Res. 256(1): 315-320.

Davis, S.G., T.A. Lyerla. 1997. The effect of lysosomotropic amines on beige mouse cells. Exp. Cell. Res. 237(1): 242-245.

Gama Sosa, M., R. De Gasperi, S. Undevia, J. Yeretsian, S. Rouse, T.A. Lyerla, and E.H. Kolodny. 1996. Correction of the galactocerebrosidase deficiency in globoid cell leukodystrophy cultured cells by SL3-3 retrovirus-mediated gene transfer. Biochem. Biophys. Res. Comm. 218: 766-771.

Gow, J.-B., S. Lainwala, and T.A. Lyerla. 1996. Enlarged dysmorphic lysosomes in an established beige (C57BL/;bgÍ/bgÍ) mouse mutant fibroblast line: A reversible characteristic. In Vitro Cell Develop. Biol. 32A: 457-461.

Gross, S.K., T.A. Lyerla, J.E. Evans, and R.H. McCluer. 1994. Expression of glycosphingolipids in serum-free primary cultures of mouse kidney cells: Male-female differences and androgen sensitivity. Molec. Cell. Biochem. 137: 25-31.

Gow, J.-B., S. Lainwala, and T.A. Lyerla. 1993. Cellular expression of the beige mouse mutation and its correction in hybrids with control human fibroblasts. In Vitro Cell Develop. Biol. 29A: 884-891.

Gross, S.K., T.A. Lyerla, M.A. Williams, and R.H. McCluer. 1992. The accumulation and metabolism of glycosphingolipids in primary kidney cell cultures from beige mice. Mol. Cell. Biochem. 118: 61-66.